Volume 5 - Engraftment Monitoring

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Objectives:

  • Understand what “chimerism” refers to in the hematopoietic stem cell transplant (HSCT) setting
  • Recognize the range of technologies that have been used to analyze donor/recipient chimerism in stem cell transplant patients
  • Understand how Short Tandem Repeat (STR) genetic loci are used in engraftment analysis
  • Understand how capillary electrophoresis (CE) separates and detects PCR amplified STR fragments
  • Understand how chimeric mixtures are evaluated using CE data
  • Understand the ASHI standards that apply to the assay processes and the quality control systems employed in testing
  • Understand common problems encountered with the use of an STR/CE system for HSCT engraftment monitoring