Volume 3 - Antibody Detection by ELISA

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Objectives:

  • Understand and identify the components of the ELISA antibody identification assay including the target antigens, secondary antibodies, enzyme substrates and their by-products.
  • Identify a positive or negative reaction, understand how this determination was obtained, and how it applies to the clinical assessment of the transplant candidate.
  • Understand and identify the ASHI standards that pertain to the compliance of the assay, the plate reader and automated washer, and the quality control measures incorporated in the test system.
CECs Earned: 
0.15