Objectives:

• Understand how to quantify extracted genomic DNA (dsDNA) and determine its purity using either UV absorbance spectroscopy or intercalating fluorochromes assays.
• Understand the contrast between the two above methods and discuss the quantity and purity requirement for diverse downstream molecular testing.
• Understand and identify the ASHI standards that pertain to the compliance of the assay, the spectrophotometer and fluorometer, and the quality control measures incorporated in the test system.