Educational Activities

E-Learning Modules

Objectives:

  • Identify the organs that are home to immune cells
  • Describe the various cells arising from a hematopoietic stem cell and their characteristics 

CEUs Earned: 0.15

Objective:

  • List important cells and tissues of the immune system and describe how cell types are distinguished from one another.

CEUs Earned: 0.15

Objective:

  • Diagram the structure of an antibody molecule and label the regions
  • List the immunoglobulin classes/isotypes and subclasses

CEUs Earned: 0.15

Objectives:

  • Define the complement system
  • List the three complement pathways

CEUs Earned: 0.15

Objectives:

  • Compare and contrast the properties of the HLA class I and class II proteins
  • Describe the role that HLA plays in immune response to pathogens

CEUs Earned: 0.15

PURPOSE OF THE MANUAL

The purpose of this manual is to provide the reader with the basic background needed to understand the HLA system and the molecular biology methods used to identify HLA "types".

CEUs Earned: 0.00

Objectives:

  • Identify the different fields in DNA nomenclature
  • Describe the relationship between HLA serology and DNA nomenclature

CEUs Earned: 0.15

Historical Highlights in Histocompatibility and Transplantation - Julius M. Cruse Jr., M.D., Ph.D.

CEUs Earned: 0.15

Objectives

Students should be able to:

•           Describe the major developments in the field of transplantation

CEUs Earned: 0.15

Objectives:

Students should be able to:

  • Describe the purpose of the International Histocompatibility Workshops
  • Describe major advances made within the Workshops

CEUs Earned: 0.15

Objectives:

  • Understand sample selection and DNA extraction procedures
  • Maximize quantity and quality of total DNA obtained in the shortest timeframe

CEUs Earned: 0.15

Objective:

  • Understand how to quantify extracted genomic DNA (dsDNA) and determine its purity using either UV absorbance spectroscopy or intercalating fluorochromes assays.

CEUs Earned: 0.15

Objectives:

  • Understand how to prepare a gel.
  • Understand how to perform gel electrophoresis.

CEUs Earned: 0.15

Objectives:

  • Describe what primers are and how they are used for HLA typing
  • Understand assay conditions that influence the specificity and sensitivity of primer-based assays

CEUs Earned: 0.15

Objective:

  • Understand and identify the ASHI standards that pertain to the maintenance and monitoring of the PCR environment.

CEUs Earned: 0.15

Objectives:

  • Describe what DNA probes are and how they are used for HLA typing
  • Understand assay conditions that influence the specificity and sensitivity of probe hybridization

CEUs Earned: 0.15

Objectives:

  • Understand how the SSP assay for HLA typing works
  • Understand and identify the ASHI standards that pertain to DNA amplification based typing.

CEUs Earned: 0.15

Objectives:

  • Understand the basic technique used to perform rSSO testing
  • Understand how to interpret test results and provide the final answer: the patient’s HLA typing result

CEUs Earned: 0.15

Objectives:

  • Understand the basic protocol for reverse sequence specific oligonucleotide (rSSO) probing for HLA typing.
  • Understand the science behind the protocols.

CEUs Earned: 0.15

Objectives:

  • Explain how DNA sequencing with chain terminators works
  • List the various strategies for sequencing HLA and give their pros and cons

CEUs Earned: 0.15

Objectives:

  • Describe newer methods of genomic DNA sequencing
  • Compare Next Generation Sequencing (NGS) to Sanger sequencing

CEUs Earned: 0.15

Objectives:

  • Describe how novel HLA alleles are first observed and characterized in the laboratory
  • Describe how novel sequences are assigned an allele designation
     

CEUs Earned: 0.15

Objective:

  • Understand various cell isolation techniques & appropriate uses for specific assays

CEUs Earned: 0.15

Objectives:

  • Describe the purpose and applications of cell cryopreservation in histocompatibility testing.
  • Identify factors that affect the viability of thawed cells.

CEUs Earned: 0.15

Objective:

  •  To understand the clinical rationale for using various types of serum treatments for HLA antibody screening and/or crossmatching by the CDC method.

CEUs Earned: 0.15

Objectives:

• Recognize when interfering substances are present and affecting HLA antibody testing 

CEUs Earned: 0.15

Objectives:

  • To describe the history and development of the microlymphocytotoxicity assay and its use in serologic HLA typing.

CEUs Earned: 0.15

Objective:

  • Describe the microscopes used to read and grade serologic HLA test reactions.

CEUs Earned: 0.15

Objective:

  • Understand basic procedures & materials required to perform a FCXM of donor cells 

CEUs Earned: 0.15

Objective:

  • Understand and identify the components of the ELISA antibody identification assay including the target antigens, secondary antibodies, enzyme substrates and their by-products.

CEUs Earned: 0.15

Objective:

  • To describe the regulations that apply to histocompatibility and immunogenetics laboratories in the United States. 

CEUs Earned: 0.15

Objective:

  • Describe the purpose of a quality management system

CEUs Earned: 0.15

Objectives:

  • Describe measurements used in the laboratory and relationships among measurements
  • Describe the math used in the laboratory to prepare reagents or to characterize results

CEUs Earned: 0.15

Objectives:
• Explain the role of killer cell immunoglobulin-like receptors in detecting the
loss of HLA class I on the cell surface
• Utilize KIR nomenclature

CEUs Earned: 0.15

Objectives:

• Obtain an understanding of the clinical problems associated with antibodies to neutrophils
• Become familiar with the human neutrophil antigen (HNA) system

CEUs Earned: 0.15

Objectives:

  • Describe the biology and genetics behind the ABO blood group
  • Determine the inheritance of ABO in a family
  • Describe the tests that determine ABO

CEUs Earned: 0.15

Objectives:

  • Understand what “chimerism” refers to in the hematopoietic stem cell transplant (HSCT) setting
  • Understand how chimeric mixtures are evaluated using CE data

CEUs Earned: 0.15

Objectives:

  • Describe the purpose and process of hematopoietic stem cell transplantation
  • List the various sources of hematopoietic stem cells

CEUs Earned: 0.15

Objectives:

  • Explain platelet refractoriness
  • Describe clinical and laboratory features
  • Describe transfusion support for platelet refractoriness

CEUs Earned: 0.15

Regional Workshop Videos

An Introduction to ASHI - Rebecca L. Upchurch, MLT (ASCP), CHS

CEUs Earned: 0.15

The Evolution of Risk Assessment in Solid Organ Transplantation - Kelley M.K. Hitchman, MS, PhD, D(ABHI)

CEUs Earned: 0.15

ASHI Quarterly Quiz